MHC-HM415TP-100μg / 询价
MHC-HM415TP-500μg / 询价
MHC-HM415TP-500μgx2 / 询价
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PE-Labeled Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Tetramer Protein
Recombinant PE-Labeled Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. PE-labeled Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Tetramer is assembled by biotinylated monomer and PE-labeled streptavidin. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and HMTEVVRHC peptide [Accession | A0A140T913(HLA-A*02:01)&P61769(B2M)&HMTEVVRHC].
Excitation Wavelength: 488 nm / 561 nm
Emission Wavelength: 575 nm
Less than 1 EU per μg by the LAL method.
Lyophilized from 0.22 μm filtered solution in 20 mM PB, 500 mM NaCl, 0.2% BSA (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized PE-Labeled Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Tetramer, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Antibody, hFc Tag with the EC50 of 3.4 ng/ml determined by ELISA.
p53 is a tumor suppressor protein. Under stressful conditions, p53 tightly regulates cell growth by promoting apoptosis and DNA repair. When p53 becomes mutated, it loses its function, resulting in abnormal cell proliferation and tumor progression. Depending on the p53 mutation, it has been shown to form aggregates leading to negative gain of function of the protein.p53 mutant associated aggregation has been observed in several cancer tissues and has been shown to promote tumor growth.
MHC; HLA-A; P53; TP53; Antigen NY-CO-13; BCC7; FLJ92943; LFS1; TRP53
(1) Kanapathipillai M. Treating p53 Mutant Aggregation-Associated Cancer. Cancers (Basel). 2018 May 23;10(6):154. doi: 10.3390/cancers10060154. PMID: 29789497; PMCID: PMC6025594.
